This technology combines the selective binding properties of a silica membrane with the speed of microspin technology. Biological samples are first lysed and homogenized in the presence of a highly denaturing guanidine-thiocyanate–containing buffer, which immediately inactivates RNases to ensure intact RNA. Ethanol is added to provide appropriate binding conditions, and the sample is then applied to an RNApure spin-column, where the total RNA binds to the membrane and contaminants are efficiently washed away. Carrier has been used in this kit to increase micro-scale RNA precipitation. High-quality RNA is then eluted in 20-50μl water.