RT-PCR is difficult to get the full length of a gene. RACE (Rapid Amplification of cDNA Ends) allows identification of full-length cDNA, including 5’, internal, and 3’ untranslated regions (UTRs). BioTeke 3′ RACE Kit is powerful to obtain the 3’ untranslated region of a target gene when only a partial sequence of a transcribed gene is available. This kit contains almost all of the reagents for 3’ RACE. First strand cDNA synthesis is initiated at the poly (A) tail of mRNA using the 3’RACE adaptor. Then amplification is performed using the user-designed gene-specific primer (GSP) 1 (Outer primer) and 3’RACE Outer Primer. If there is no product in the first reaction, please use nested GSP2 (Inner primer) and 3’RACE Inner Primer perform second PCR. PCR products can be used for sequencing and cloning.