Innovative extraction and lysis system allow for rapidly lysing cells and inactivating cellular nuclease. The treatment of glass beads is eliminated and the integrity of genomic DNA is guaranteed. DNA purity is greatly ensured from efficiently removing debris, contaminants and humic acid by specially treated DNA spin-column. Innovative washing solution removes trace contaminants and pure DNA is eluted in water or low ionic strength Elution Buffer. Purified DNA can be directly used in downstream applications without the need for further purification.